William Gamble (1 January 1818 – 20 December 1866) was a civil engineer and a United States Army cavalry officer. He served during the Second Seminole War, and fought for the Union during the American Civil War. He commanded one of two brigades in Brigadier General John Buford's Division of Cavalry, in which he played an important role in defending Union positions during the first day of the Battle of Gettysburg.