Rapid amplification of cDNA ends (RACE) is a technique used in molecular biology to obtain the full length sequence of an RNA transcript found within a cell. RACE results in the production of a cDNA copy of the RNA sequence of interest, produced through reverse transcription, followed by PCR amplification of the cDNA copies (see RT-PCR). The amplified cDNA copies are then sequenced and, if long enough, should map to a unique genomic region. RACE is commonly followed up by cloning before sequencing of what was originally individual RNA molecules. A more high-throughput alternative which is useful for identification of novel transcript structures, is to sequence the RACE-products by next generation sequencing technologies.
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| - RACE-PCR (de)
- RACE (it)
- RACE-PCR (fr)
- RACE PCR (pl)
- Rapid amplification of cDNA ends (en)
- 快速扩增cDNA末端 (zh)
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| - Rapid amplification of cDNA ends (RACE) is a technique used in molecular biology to obtain the full length sequence of an RNA transcript found within a cell. RACE results in the production of a cDNA copy of the RNA sequence of interest, produced through reverse transcription, followed by PCR amplification of the cDNA copies (see RT-PCR). The amplified cDNA copies are then sequenced and, if long enough, should map to a unique genomic region. RACE is commonly followed up by cloning before sequencing of what was originally individual RNA molecules. A more high-throughput alternative which is useful for identification of novel transcript structures, is to sequence the RACE-products by next generation sequencing technologies. (en)
- RACE PCR (od ang. rapid amplification of cDNA ends PCR), szybka amplifikacja końców cDNA – technika biologii molekularnej, odmiana łańcuchowej reakcji polimerazy (PCR), której celem jest poznanie dokładnej sekwencji jednego z końców RNA. W zależności od analizowanego końca stosowana jest metoda 3'RACE lub 5'RACE. (pl)
- 快速扩增cDNA末端（Rapid amplification of cDNA ends，縮寫RACE）技术，指的是利用分子生物学获得RNA转录本3'或5'序列的技术。该技术是基于PCR技术，快速扩增cDNA（RNA反转录获得）末端从而获得已知mRNA内一段小序列与3'或5'序列的技术。该技术也称单侧PCR或者锚式PCR。 (zh)
- Als RACE oder RACE-PCR (englisch rapid amplification of cDNA-ends und polymerase chain reaction) wird in der Molekularbiologie eine Kombination von Methoden zur schnellen Vervielfältigung von cDNA-Enden mit Hilfe der Polymerase-Kettenreaktion bezeichnet. (de)
- RACE-PCR est l'acronyme de l'expression anglaise « rapid amplification of cDNA-ends by polymerase chain reaction » et qui signifie en français « amplification rapide d'extrémités de cDNA par réaction en chaîne par polymérase ». La première partie de l'acronyme « RACE » est un jeu de mot (« course » en français) qui fait allusion à la rapidité de la méthode. Le but de la RACE-PCR tient dans l'isolement des extrémités 3' ou 5' de gènes afin de déterminer la séquence d'initiation ou de terminaison de la traduction par exemple. C'est une technique dérivée de la RT-PCR. (fr)
- La sigla RACE è l'abbreviazione di rapid amplification of cDNA ends (in italiano: amplificazione rapida delle estremità del cDNA) ed è una tecnica di biologia molecolare che serve ad estendere la porzione di sequenza nota di un RNA messaggero all'estremità (5'-RACE) o (3'-RACE). Le classiche tecniche di clonaggio del cDNA infatti spesso producono soltanto frammenti della sequenza, che possono essere estesi tramite le tecniche di RACE. (it)
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| - Als RACE oder RACE-PCR (englisch rapid amplification of cDNA-ends und polymerase chain reaction) wird in der Molekularbiologie eine Kombination von Methoden zur schnellen Vervielfältigung von cDNA-Enden mit Hilfe der Polymerase-Kettenreaktion bezeichnet. Eine RACE-PCR ist zumeist eine Form bzw. eine Abwandlung der RT-PCR, mit der die Enden von Genen erfasst werden können. Ein Ziel der RACE besteht oft in der Erfassung eines Gens als Locus im Genom (als Abschnitt im Erbgut). Während die Start- und Endpunkte der codierenden Sequenz von Genen meist durch die DNA-Sequenz selbst erkennbar sind (z. B. mit Hilfe von offenen Leserahmen), lassen sich die untranslatierten Regionen (UTRs) an den beiden Enden eines Gens nicht direkt aus der Genomsequenz ableiten. Die untranslatierten Regionen enthalten jedoch wichtige regulatorische Elemente, die die Translation der mRNA und deren Stabilität beeinflussen und sind daher von großem Interesse. (de)
- Rapid amplification of cDNA ends (RACE) is a technique used in molecular biology to obtain the full length sequence of an RNA transcript found within a cell. RACE results in the production of a cDNA copy of the RNA sequence of interest, produced through reverse transcription, followed by PCR amplification of the cDNA copies (see RT-PCR). The amplified cDNA copies are then sequenced and, if long enough, should map to a unique genomic region. RACE is commonly followed up by cloning before sequencing of what was originally individual RNA molecules. A more high-throughput alternative which is useful for identification of novel transcript structures, is to sequence the RACE-products by next generation sequencing technologies. (en)
- RACE-PCR est l'acronyme de l'expression anglaise « rapid amplification of cDNA-ends by polymerase chain reaction » et qui signifie en français « amplification rapide d'extrémités de cDNA par réaction en chaîne par polymérase ». La première partie de l'acronyme « RACE » est un jeu de mot (« course » en français) qui fait allusion à la rapidité de la méthode. Le but de la RACE-PCR tient dans l'isolement des extrémités 3' ou 5' de gènes afin de déterminer la séquence d'initiation ou de terminaison de la traduction par exemple. C'est une technique dérivée de la RT-PCR.
* Portail de la biologie cellulaire et moléculaire (fr)
- La sigla RACE è l'abbreviazione di rapid amplification of cDNA ends (in italiano: amplificazione rapida delle estremità del cDNA) ed è una tecnica di biologia molecolare che serve ad estendere la porzione di sequenza nota di un RNA messaggero all'estremità (5'-RACE) o (3'-RACE). Le classiche tecniche di clonaggio del cDNA infatti spesso producono soltanto frammenti della sequenza, che possono essere estesi tramite le tecniche di RACE. Il metodo si basa sull'uso della reazione a catena della polimerasi; il problema maggiore è quindi quello di individuare due primer ai lati della sequenza da amplificare. Uno dei primer può essere basato sulla sequenza nucleotidica già nota, che deve venire estesa. Il secondo primer invece è più problematico e tecniche diverse si utilizzano, come si vedrà nella sezione successiva, per le estremità 3' e 5'. L'uso della sigla RACE è anche un gioco di parole, in quanto in inglese il termine race significa corsa; corsa appunto in una o nell'altra direzione della sequenza nucleotidica. (it)
- RACE PCR (od ang. rapid amplification of cDNA ends PCR), szybka amplifikacja końców cDNA – technika biologii molekularnej, odmiana łańcuchowej reakcji polimerazy (PCR), której celem jest poznanie dokładnej sekwencji jednego z końców RNA. W zależności od analizowanego końca stosowana jest metoda 3'RACE lub 5'RACE. (pl)
- 快速扩增cDNA末端（Rapid amplification of cDNA ends，縮寫RACE）技术，指的是利用分子生物学获得RNA转录本3'或5'序列的技术。该技术是基于PCR技术，快速扩增cDNA（RNA反转录获得）末端从而获得已知mRNA内一段小序列与3'或5'序列的技术。该技术也称单侧PCR或者锚式PCR。 (zh)
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