The Jonesborough Historic District is a historic district in Jonesborough, Tennessee, that was listed on the National Register of Historic Places as Jonesboro Historic District (reflecting the spelling of the town name at the time) in 1969. Staff of the Tennessee Historical Commission surveyed 158 buildings in the town in 1969 and found 72 worth preservation. The district includes Queen Anne, Greek Revival, and Federal styles of architecture among its 72 contributing buildings located in a more than 120-acre (49 ha) area.