Daniel F. Desdunes (c. 1870 – April 24, 1929) was a civil rights activist and musician in New Orleans and Omaha, Nebraska. Descended from a family of people of color free before the Civil War, in 1892 he volunteered to board a train car designated for whites in violation of the Louisiana 1890 Separate Car Act. This would be a test case to enable the New Orleans Comité des Citoyens to challenge the law in the courts. The train he boarded was an interstate train, and the court found that the law did not apply to such cases, which were bound by federal law and regulation.