The 1959 Wyoming Cowboys football team represented the University of Wyoming in the Skyline Conference during the 1959 NCAA University Division football season. In their third season under head coach Bob Devaney, the Cowboys compiled a 9–1 record (7–0 against Skyline opponents), won the Skyline Conference championship, and outscored all opponents by a total of 287 to 62. The Cowboys were not ranked during the season, but after the season concluded they were ranked No. 16 in the final AP Poll.